解剖学报

›› 2009, Vol. 40 ›› Issue (6): 933-937.doi: 10.3969/j.issn.0529-1356.2009.06.017

• 论著 • 上一篇    下一篇

肝细胞核因子-1α和肝细胞核因子-4α在人肝细胞癌中的表达及意义

郑洁1 ;董承伟2 ;蒋吉英3*; 张皓云3 ;魏德全3 ;丁洁3   

  1. 1.潍坊医学院病理学教研室，山东潍坊 261042; 2. 潍坊市人民医院肝胆外科，山东潍坊 261042;3.潍坊医学院解剖学教研室，山东潍坊 261042
  • 收稿日期:2008-10-06 修回日期:2008-11-27 出版日期:2009-12-06
  • 通讯作者: 蒋吉英

Expressions of hepatocyte nuclear factor -1α and hepatocyte nuclear factor -4α in human hepatocellular carcinoma

  1. 1.Department of Pathology, Weifang Medical College, Shandong Weifang 261042, China; 2. Department of Hepatobiliary Surgery, Weifang People’s Hospital, Shandong Weifang 261042, China; 3. Department of Anatomy, Weifang Medical College, Shandong Weifang 261042, China
  • Received:2008-10-06 Revised:2008-11-27 Online:2009-12-06
  • Contact: JIANG Ji-ying

关键词: HNF-1α基因, HNF-4α基因, 肝细胞癌, RT-PCR, 免疫组织化学, 人

Abstract: Objective To investigate the expression of hepatocyte nuclear factor-1α (HNF-1α) and hepatocyte nuclear factor-4α (HNF-4α) in human hepatocellular carcinoma (HCC) and explore the function of HNF-1α and HNF-4α during HCC carcinogenesis and development. Methods Twenty-six specimens of hepatocellular carcinoma were collected. The expressions of HNF-1α and HNF-4α in HCC tissues and adjacent non-cancerous tissues were detected by reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemistry staining. Results The mRNA levels of HNF-1α and HNF-4α were significantly lower in HCC tissues than that in adjacent non-cancerous tissues (0.818±0.371 vs. 0.383±0.102 for HNF-1α, P0.05; 0.846±0.384 vs. 0.397±0.105 for HNF-4α, P0.05）.The positive rates of HNF-1α and HNF-4α protein were significantly lower in HCC tissues than in adjacent non-c

Key words: HNF-1α, HNF-4α, Hepatocellular carcinoma, RT-PCR, Immunohistochemistry, Human

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